Robust, Ready-to-Use Nuclear Receptor Assay Systems
You want results, and you want them fast. INDIGO is 100% focused on Nuclear Receptor assay development and screening, so you can focus on your research. Our complete INDIGO Nuclear Receptor Assay Systems are ready-to-use when you are ready to test.
INDIGO Biosciences brings our Nuclear Receptor expertise directly into your lab via our easy-to-use assay kits. Available for shipment on demand, the all-inclusive assay systems feature proprietary non-human mammalian cells engineered to provide constitutive, high-level expression of Nuclear Receptors. Because these cells incorporate a responsive luciferase reporter gene, quantifying resulting luciferase activity provides a sensitive surrogate measure of Nuclear Receptor activity in treated cells, ideal for quantifying inverse-agonist or antagonist activities against Nuclear Receptors. Each kit includes Nuclear Receptor reporter cells, microplate (96- or 384-well), all required reagents (optimized culture media, positive-control agonist, and detection reagent), as well as a detailed Technical Manual and Protocol Quick Guide.
Advantages of INDIGO Nuclear Receptor Assays:
- Thaw and Use: Data in 24 hours
- ≥ 95% viability post-thaw
- No need for intermediate spin-and-wash steps, viability determinations, or cell titer adjustments
- Stable light emission over at least 1 hour
- Excellent Z’ and Signal to Background scores
- Flexible formats for low-, medium-, and high-throughput screening
- Streamlined protocol optimized for Automation
Thaw. Feed. Dose. Read.™
Quickly and accurately measure Retinoid Receptor activity using INDIGO Biosciences cell-based assay kits
The Retinoid Receptors are an important class of Nuclear Receptors. There are three Retinoid Receptors, each with three Subtypes. They are: Retinoic Acid Receptor (RARalpha, RARbeta, RARgamma, RAR panel); Retinoic Orphan Receptor (RORalpha, RORbeta, RORgamma); and Retinoid X Receptor (RXRalpha, RXRbeta, RXRgamma). INDIGO Biosciences has complete cell-based assay systems available for several of the Retinoid Receptors, including RORgamma/NR1F3.