Promo GeneDireX
GeneDireX established their brand in 2007 and is focused on providing innovative products to the scientific community worldwide. They take advantage of cutting-edge technologies to provide research tools that are high quality, affordable and easy to use. They are the largest DNA and Protein Ladders manufacturer, providing reliable molecular ladders to researchers globally through their channel partners and sales offices.
Description
A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 11 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 100-1,500 base pairs. The 500 and 1,500 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples of similar size.
Description
A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and PCR products to yield 13 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 250-10,000 base pairs. The 1K and 3K bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.5 μg a load) for approximating the mass of DNA in comparably intense samples of similar size.
Description
A unique combination of PCR products and a number of proprietary plasmids digested with appropriate restriction enzymes to yield 17 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 50-1,500 base pairs. The 200, 500 and 1200 base pair bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.56 μg a load) for approximating the mass of DNA in comparably intense samples of similar size.
At 300V, 3000mA, 300W, the PowerPro 3AMP is designed for virtually all high current electrophoresis applications. The PowerPro 3AMP’s higher current output capability is perfect for electroblotting units with high-intensity plate electrodes, particularly Cleaver Scientific omniBLOT maxi, VS20 WAVE and semi dry blotting systems.
Description
A unique combination of a number of proprietary plasmids digested with appropriate restriction enzymes and PCR products to yield 14 fragments, suitable for use as molecular weight standards for agarose gel electrophoresis. The DNA includes fragments ranging from 250-25K base pairs. The 1K and 3K bands have increased intensity to serve as reference points. The approximate mass of DNA in each band is provided (0.52 μg a load) for approximating the mass of DNA in comparably intense samples of similar size.
The BLUltra Prestained Protein Ladder is a three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 6.5 to 270 kDa. Proteins are covalently coupled with a blue chromophore except for three reference bands (two orange bands at 30 kDa and 270 kDa and one green band at 52 kDa) when separated on SDS-PAGE (Tris-glycine buffer). The BLUltra Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in the gel loading buffer and is ready to use.
The BLUeye Prestained Protein Ladder is a three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BLUeye Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use.
The BLUelf Prestained Protein Ladder is a three-color protein standard with 13 pre-stained proteins covering a wide range molecular weights from 3.5 to 245 kDa. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-glycine buffer). The BLUelf Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.
The PiNK Plus Prestained Protein Ladder contains 11 proteins that resolve into sharp, tight bands in the range of 10-175 kDa. The PiNK Plus Prestained Protein Ladder allows you to monitor molecular weight separation during electrophoresis, estimate molecular weights of proteins of interest, and evaluate western transfer efficiency.
Nimble Juice is a fast and sensitive fluorescent dye for visualization and quantitation of proteins separated by 1-D or 2-D SDS-PAGE. It comes as a 100x stock solution that is simply diluted with water by the user to its working concentration. Nimble Juice is normally low fluorescent but emits strong fluorescence (bright golden color) as bound to proteins. The staining procedure is a simple two-step protocol (fix and stain) that can be completed in as little as 30 minutes. Gels to be stained are fixed with ethanol/acetic acid solution prior to staining with Nimble Juice solution. A destain step is not normally recommended, but may be employed to reduce background, simply by agitating the gel in water for 1-5 minutes. Gels stained with Nimble Juice fluorescent gel stain may be directly visualized with a variety of different UV-based fluorescence imaging systems. The maximum emission wavelength of protein-bound Nimble Juice is near 570 nm. Nimble Juice gives exceptional sensitivity and wide dynamic range for protein detection. The bound Nimble Juice dye is easily removed from the protein by immersing the gel in sufficient water, thus it is well compatible with subsequent enzymatic digestion and mass spectrometry for proteomics applications. Stained gels may be stored in stain solution in the dark at 2-8°C; imaging sensitivity might be moderately enhanced after 4°C storage of the stained gel.
Novel Juice is a non-mutagenic fluorescent reagent that produces instant visualization of DNA bands upon Blue Light or UV illumination of agarose gels. Supplied in GeneDireX’s 6X DNA Loading Buffer, Novel Juice is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. Novel Juice is the most sensitive stain available for detecting the double-stranded DNA (dsDNA). It contains three tracking dyes (Bromophenol Blue, Xylene Cyanol FF, and Orange G) for visually tracking the DNA migration during the electrophoresis process. It is ideal for the environment requiring a safe , non-hazardous alternative to Ethidium Bromide.
10 mM dNTP (2′-deoxynucleoside 5′-triphosphate) Mix consists of all four nucleotides (dATP, dCTP, dGTP, dTTP), each at a concentration of 10 mM, in a solution of 0.6 mM Tris-HCl (pH 7.5). The 10 mM dNTP Mix is suitable for use in polymerase chain reaction (PCR), sequencing, fill-in, nick translation, cDNA synthesis, and TdT-tailing reactions.
Taq DNA Polymerase is a simple, specificity PCR reaction mixture. Simply add primers, template, dNTPs, and PCR grade water, the reagent will execute primer extensions and other molecular biology applications. Taq DNA Polymerase contains two components, include the Taq DNA polymerase and 10X PCR buffer.
The Taq DNA Polymerase is purified from E coli., expressing a Thermus aquaticus DNA polymerase gene. This enzyme has a 5′ →3′ DNA polymerase and a 5′ → 3′ exonuclease activity but lacks a 3′ → 5′ exonuclease activity. The enzyme consists of a single polypeptide with a mo lecular weight of approximately 94 kilo Dalton. Taq DNA polymerase is heat-stable and will synthesize DNA at eleva ted temperatures from single-stranded templates in the presence of a primer. The Taq DNA Polymerase is recommended for use in routine PCR reactions. The 10X PCR buffer is optimized for high specificity and guarantees minimal by product formation. Usually 1-1.5 unit of Taq DNA polymerase is used in 50 μl of reaction mix. Higher Taq DNA polymerase concentrations may cause synthesis of nonspecific products. However, if inhibitors exist in the reaction mixture (e.g., if the template DNA used is not highly purified), higher amounts of Taq DNA polymerase (2-3 units) may be necessary to obtain a better yield of amplification products.
OnePCR is a ready-to-use PCR reaction mixture. Simply add primers, template, and water, the reagent will execute primer extensions and other molecular biology applications. OnePCR is a pre-mixed solution containing Taq DNA polymerase, PCR Buffer, dNTP, gel loading dyes, and fluorescence dye.
OnePCR which contains the Taq DNA polymerase, is purified from the E. coli., and expressing the Thermus aquaticus DNA polymerase gene. This enzyme has a 5′ → 3′ DNA polymerase and the 5′ → 3′ exonuclease activity but lacks the 3′ → 5′ exonuclease activity. OnePCR, which contains the fluorescence dye, is directly detected on BLooK LED transilluminator or UV epi-illuminator after the DNA electrophoresis. OnePCR mixture is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions.
Reagents are provided with the sufficient amplification reactions of 50 μl each.
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