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Discover Protein A and Protein G Magnetics Beads Agarose Beads (25%). These beads are coated with agarose and have a superparamagnetic core, which allows a simple, fast and efficient method for antibody capture and purification. These products are used in downstream processes for immunoprecipitation (IP), co-immunoprecipitation(Co-IP), chromatin immunoprecipitation(ChIP), and small-scale IgG purifications.
ABT also added Streptavidin 6HC Agarose Resin to our catalog. This product has been developed to perform purification of biotinylated biomolecules. Streptavidin is a tetrameric protein containing four biotin-binding sites and is immobilized on a highly crosslinked beaded agarose.
Nickel Rapid Run™
Nickel chelate resins recognize two exposed histidines for an appreciable retention of a protein and it is recommended in the majority of the cases. This resin works well with relatively easy to separate His-tagged proteins. Nickel Rapid Run™ provides the advantages of the Nickel cation with the high flow rates of the Rapid Run™ beads. It is specially designed for large scale His-tagged protein purifications.
| Product Name | Nickel Rapid Run™ |
| Cat. No. | 6NiRR-X |
| Bead Geometry & Size | Spherical,Standard ~ 50-150 µm |
| Crosslinked | Highly crosslinked |
| Agarose % | 6% |
| Maximum Flow Rate at 15 cm Bed Height | ≥ 1000 cm/h |
| Maximum Pressure at 15 cm bed Height | ≥ 300 kPa |
| Exclusion Limit Da | ~4×10^6 |
| Ligand | Iminodiacetic Acid |
| Loading Capacity (μmol me2+/ml gel) | ~20 |
| Antimicrobial Agent | 20% Ethanol |
| Storage Temperature | 2-8˚C |
Glutathione Agarose Resin
Glutathione Agarose Resin consists of glutathione covalently bound to agarose beads. This resin works well with small and large proteins tagged with GST in batch or column purifications (Low Flow).
| Product Name | Glutathione Agarose Resin |
| Cat. No. | 4B-GLU-X |
| Bead Geometry & Size | Spherical, Standard ~ 50-150 µm |
| Crosslinked | No |
| Agarose % | 4% |
| Ligand | Glutathione, linked via sulphur atom |
| Static Binding Capacity | ≥ 8 mg recombinant GST / ml gel |
| Storage Temperature | 4-8 C° |
Protein G Agarose Resin 4 Rapid Run™
Protein G Agarose Resin 4 Rapid Run™ consists of rProtein G covalently bound to highly crosslinked agarose beads. It provides a very stable bond that can greatly minimize leakage of the Protein G allowing for reuse of the affinity resin in several purification steps. The resin works well in High Flow conditions.
| Product Name | Protein G Agarose Resin 4 Rapid Run™ |
| Cat. No. | 4RRPG-X |
| Bead Geometry & Size | Spherical,Standard: ~50-150 µm |
| Crosslinked | Highly crosslinked |
| Agarose % | 4% |
| Coupling Method | Coupling binding by reductive amination |
| Static Binding Capacity | ~20 mg human IgG/ ml resin |
| Antimicrobial Agent | 20% Ethanol |
| Storage Temperature | 2-8˚C |
Sepadextran™-50 Fine
Sepadextran™ is a beaded gel filtration medium prepared by crosslinking dextran and it is supplied in its dry form. Sepadextran™-50 can be used for protein and nucleic acid purifications being the exclusion limit 25 kD for proteins and 20 bases for nucleic acids.
Fine grade are for preparative separations and routine laboratory work.
| Product Name | Sepadextran™ 50 Fine |
| Cat. No. | SD50F-X |
| Matrix | Crosslinked dextran |
| Water Regain | 4.80-5.20 ml/g |
| Swelling | 9-11 ml/g |
| Dry Particle Size | 20-80 µm (>80%) |
| Wet Particle Size | 40-160 µm |
| Maximum Operating Pressure | Generally obeys Darcy’s Law: U=Ko ΔP/L Where: U= linear flow rate(cm/h). ΔP= pressure drop over gel bead (cm H2O). L= bed high(cm) Ko=36 |
| Fractionation Range | 1.5-30 kD for globular proteins, 0.5-10 kD for dextrans |
| pH Stability | 2.0 to 13 |
| Autoclavable | 121˚C, pH 7.0 (30 minutes) |
| Chemical Stability | All commonly used buffers, including: 0.1 M NaOH; 0.01 M HCl; 1 M acetic acid; 8 M urea; 6 M guanidine HCl; 1% SDS, 24% Ethanol; 30% Propanol; 30% Acetonitrile |
| Storage Temperature | Ambient |
